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Structured Review

Difco yeast transformants
Yeast Transformants, supplied by Difco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yeast+transformants/transformants+yeast/pmc13156747-214-4-13
Average 86 stars, based on 1 article reviews
yeast transformants - by Bioz Stars, 2026-09
86/100 stars

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Incubation:

Article Title: High level expression of the major core protein VP7 and the non-structural protein NS3 of bluetongue virus in yeast: use of expressed VP7 as a diagnostic, group-reactive antigen in a blocking ELISA.
Article Snippet: The major core protein VP7 and a non-structural protein NS3 of bluetongue virus serotype 1 have been synthesized from recombinant plasmids using both an in vitro transcription/ translation system and a yeast expression system.. Bluetongue virus genes were transcribed under the control of the bacteriophage SP6 promoter and the regulatable yeast metallothionein promoter.. An indirect ELISA showed that expression of NS3 in yeast was inducible with 1 mM CuSO, and VP7 synthesis was constitutive but could be further induced.

Functional Assay:

Article Title: Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors
Article Snippet: Uracil auxotrophic yeast strains were selected by 5-fluoroorotic acid (5-FOA) medium containing 6.7 g L −1 YNB with ammonium sulphate and without amino acids, 20 g L −1 glucose, 1 g L −1 5-FOA (Zymo Research, USA) and essential amino acids as well as uracil. .. For the functional complementation, Western blot analysis and two step quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments, the yeast transformants were induced for hCAII expression by cultivation in synthetic galactose (SG) medium (6.7 g L −1 YNB without amino acids, 10 g L −1 raffinose, 20 g L −1 galactose; Difco, USA) containing 0.2 g L −1 adenine, 1 g L −1 leucine and 0.1 g L −1 histidine (SG + Ade + Leu + His). .. The Δ nce103 strain was grown under the high-CO 2 condition (5% (v/v) CO 2 ) using an AnaeroPack (Mitsubishi Gas Chemical, Japan).

Western Blot:

Article Title: Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors
Article Snippet: Uracil auxotrophic yeast strains were selected by 5-fluoroorotic acid (5-FOA) medium containing 6.7 g L −1 YNB with ammonium sulphate and without amino acids, 20 g L −1 glucose, 1 g L −1 5-FOA (Zymo Research, USA) and essential amino acids as well as uracil. .. For the functional complementation, Western blot analysis and two step quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments, the yeast transformants were induced for hCAII expression by cultivation in synthetic galactose (SG) medium (6.7 g L −1 YNB without amino acids, 10 g L −1 raffinose, 20 g L −1 galactose; Difco, USA) containing 0.2 g L −1 adenine, 1 g L −1 leucine and 0.1 g L −1 histidine (SG + Ade + Leu + His). .. The Δ nce103 strain was grown under the high-CO 2 condition (5% (v/v) CO 2 ) using an AnaeroPack (Mitsubishi Gas Chemical, Japan).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors
Article Snippet: Uracil auxotrophic yeast strains were selected by 5-fluoroorotic acid (5-FOA) medium containing 6.7 g L −1 YNB with ammonium sulphate and without amino acids, 20 g L −1 glucose, 1 g L −1 5-FOA (Zymo Research, USA) and essential amino acids as well as uracil. .. For the functional complementation, Western blot analysis and two step quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments, the yeast transformants were induced for hCAII expression by cultivation in synthetic galactose (SG) medium (6.7 g L −1 YNB without amino acids, 10 g L −1 raffinose, 20 g L −1 galactose; Difco, USA) containing 0.2 g L −1 adenine, 1 g L −1 leucine and 0.1 g L −1 histidine (SG + Ade + Leu + His). .. The Δ nce103 strain was grown under the high-CO 2 condition (5% (v/v) CO 2 ) using an AnaeroPack (Mitsubishi Gas Chemical, Japan).

Expressing:

Article Title: Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors
Article Snippet: Uracil auxotrophic yeast strains were selected by 5-fluoroorotic acid (5-FOA) medium containing 6.7 g L −1 YNB with ammonium sulphate and without amino acids, 20 g L −1 glucose, 1 g L −1 5-FOA (Zymo Research, USA) and essential amino acids as well as uracil. .. For the functional complementation, Western blot analysis and two step quantitative reverse transcription-polymerase chain reaction (qRT-PCR) experiments, the yeast transformants were induced for hCAII expression by cultivation in synthetic galactose (SG) medium (6.7 g L −1 YNB without amino acids, 10 g L −1 raffinose, 20 g L −1 galactose; Difco, USA) containing 0.2 g L −1 adenine, 1 g L −1 leucine and 0.1 g L −1 histidine (SG + Ade + Leu + His). .. The Δ nce103 strain was grown under the high-CO 2 condition (5% (v/v) CO 2 ) using an AnaeroPack (Mitsubishi Gas Chemical, Japan).

Article Title: Golgi-derived phosphatidylinositol 4-phosphate is crucial for organization of the preautophagosomal structure
Article Snippet: .. To maintain plasmid expression, yeast transformants were cultured in SD/CA medium (0.17% w/v Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% w/v ammonium sulfate, 0.5% w/v Bacto casamino acids, and 2% w/v glucose) or SD/DO medium (0.17% w/v Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% w/v ammonium sulfate, 2% w/v glucose), each supplemented with the appropriate amino acids. ..

Plasmid Preparation:

Article Title: Golgi-derived phosphatidylinositol 4-phosphate is crucial for organization of the preautophagosomal structure
Article Snippet: .. To maintain plasmid expression, yeast transformants were cultured in SD/CA medium (0.17% w/v Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% w/v ammonium sulfate, 0.5% w/v Bacto casamino acids, and 2% w/v glucose) or SD/DO medium (0.17% w/v Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% w/v ammonium sulfate, 2% w/v glucose), each supplemented with the appropriate amino acids. ..

Cell Culture:

Article Title: Golgi-derived phosphatidylinositol 4-phosphate is crucial for organization of the preautophagosomal structure
Article Snippet: .. To maintain plasmid expression, yeast transformants were cultured in SD/CA medium (0.17% w/v Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% w/v ammonium sulfate, 0.5% w/v Bacto casamino acids, and 2% w/v glucose) or SD/DO medium (0.17% w/v Difco yeast nitrogen base without amino acids and ammonium sulfate, 0.5% w/v ammonium sulfate, 2% w/v glucose), each supplemented with the appropriate amino acids. ..



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